Liu, Qing Sun, Shujing Piao, Meizi Yang, Ji Young Purification and Characterization of a Protease Produced by a Planomicrobium sp. These results begin to address the diversity of protease-producing bacteria and bacterial extracellular proteases in the sediments of the Antarctic Sea. Inhibitor analysis showed that nearly all the extracellular proteases from the bacteria were serine proteases or metalloproteases. Among these strains, Pseudoalteromonas and Flavobacteria showed relatively high protease production. The cultivated protease-producing bacteria were mainly affiliated with the phyla Actinobacteria, Firmicutes, Bacteroidetes, and Proteobacteria, and the predominant genera were Bacillus (22.9%), Flavobacterium (21.0%) and Lacinutrix (16.2%). The cultivable protease-producing bacteria reached 105 cells/g in all 8 sediment samples. In this paper, the diversity of the cultivable protease-producing bacteria and of bacterial extracellular proteases in the sediments of Maxwell Bay, King George Island, Antarctica was investigated. However, the diversity of these bacteria and their extracellular proteases in most regions remain unknown. Protease-producing bacteria play a vital role in degrading sedimentary organic nitrogen. Zhou, Ming-Yang Wang, Guang-Long Li, Dan Zhao, Dian-Li Qin, Qi-Long Chen, Xiu-Lan Chen, Bo Zhou, Bai-Cheng Zhang, Xi-Ying Zhang, Yu-Zhong In this study, each of the biotechnologically valuable alkaline protease producing isolates was grouped into their taxonomic positions with multi-genotypic analyses.ĭiversity of Both the Cultivable Protease-Producing Bacteria and Bacterial Extracellular Proteases in the Coastal Sediments of King George Island, Antarctica While ITS-PCR provided the highest correlation with 16S rRNA groups, (GTG)5-PCR showed the highest differentiation at species and intra-species level. These different techniques allowed us to differentiate the alkaliphilic isolates and the results were in concurrence with phylogenetic analyses of the 16S rRNA genes.
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Genotypic characterizations of the isolates were also determined by using a wide range of molecular techniques (ARDRA, ITS-PCR, (GTG)5-PCR, BOX-PCR). Interestingly, the isolates identified as Bacillus safensis were also found to be high alkaline protease producing strains. The highest alkaline protease producing isolates (103125 U/g), E114 and C265, were identified as Bacillus licheniformis with 99.4% and Bacillus mojavensis 99.8% based on 16S rRNA gene sequence similarities, respectively.
Seven isolates were showed higher alkaline protease production capacity than the reference strains. 56 bacteria, capable of growing under alkaline conditions were isolated and their alkaline protease activities were carried out at different parameters to determine their optimum alkaline protease production conditions. Tekin, Nilgun Cihan, Arzu Coleri Karaca, Basar Cokmus, CumhurĪlkaline proteases have biotechnological importance due to their activity and stability at alkaline pH. A Comparative Study: Taxonomic Grouping of Alkaline Protease Producing Bacilli.